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KMID : 0371320010610030305
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Journal of the Korean Surgical Society
2001 Volume.61 No. 3 p.305 ~ p.311
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Microsatellite Alterations of Chromosome 9p, 13q, 16q in Hepatocellular Carcinoma
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Cho Seong-Jin
Kim Nam-Ryeol
Min Youn-Ki
Joh Yong-Geul
Cho Min-Young
Seo Seong-Ok
Yeom Bom-Woo
Won Nam-Hee
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Abstract
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Purpose
Hepatocellular carcinoma (HCC) patients are asymptomatic and the tumor remains in an unresectable state until the tumor progresses. Recently much efforts for elucidation of the early hepatocarcinogenesis have been made, and for this purpose it is
very
crucial to investigate the genetic abnormalities. We evaluated microsatellite alterations of five markers from chromosome 9, 13, 16 and investigated the relationships with the clinicopathological parameters in HCC.
Methods
The microsatellite alteration analysis was performed using polymerase chain reaction with five polymorphic microsatellite markers (D9S171, D9S1747, D13S156, D16S419, D16S3106) in 40 surgically resected HC
Cs and their respective non-tumorous counterparts.
Results
D9S171, D9S1747, D13S156, D16S419, D16S3106 abnormalities were detected in 20.0%, 14.3%, 50.0%, 32.4% and 22.6%, respectively. Loss of heterozygosity (LOH) of D9S171 correlated well with higher tumor histologic grade and LOH of D13S156, D16S419
and
D16S3106 correlated well with increased tumor size. Microsatellite instability (MSI) was found in two markers, D13S156, D16S419.
Conclusion
As a result, we concluded that alterations in microsatellites of various chromosomes may contribute to the hepatocarcinogenesis and tumor progression. Especially LOH of chromosome 13 and 16 are considered to correlate with tumor progression.
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KEYWORD
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